Journal: Journal of Translational Medicine

Article Title: Intranasally administered muse cells attenuate neurodegeneration in Parkinson’s disease

doi: 10.1186/s12967-025-07401-6

Figure Lengend Snippet: Muse cell has protective effects on DA neurons. A : (200×magnification) Compared to normal WT mice, the PBS group showed reduced TH and NeuN staining in the SNc region. In contrast to the PBS group, the muse group exhibited a higher number of TH-positive cells and NeuN-positive cells, with PKH26-labeled muse cells present in the region. Compared to the muse group, the muse + J and non-muse groups displayed relatively fewer TH-positive cells( n = 6). B : S1P expression in the brain tissue of A53T mice (PBS group) was significantly increased compared with normal WT mice. In the group treated with muse cells, S1P expression was markedly reduced relative to both the PBS and non-muse groups, though it remained higher than that in normal WT mice. Western blot results indicated: TNF-α expression in the brains of the PBS group was significantly higher than in control. Compared to the PBS group, TNF-α expression was significantly reduced in the muse group, while the non-muse and muse + J groups also showed reduced TNF-α expression. TNF-α expression in the muse group was lower than in the non-muse and muse + J groups. Expression levels of BDNF and GDNF showed a marked decline in the PBS group. No significant change was seen with non-muse cell treatment, whereas the muse group displayed elevated expression. The expression remained unchanged in the muse + J group. (*Compared to the control group, ** P < 0.01, and compared to the PBS group, # P < 0.05, ## P < 0.01, & compared to the muse group, & P < 0.05) ( n = 3). C : The H&E staining results (200× magnification) showed that in the substantia nigra region of normal WT mice, the tissue structure was normal, and the cell nuclei were clearly visible (red arrows indicate astrocytes, green arrows indicate neuronal cells). In contrast, the brains of PBS group mice exhibited significant neuronal loss, with abnormal neuronal nuclear morphology and blurred boundaries. The non-muse group and muse + J group showed similar conditions to the PBS group, but the degree of neuronal damage was reduced. In the muse group, the number of damaged neurons was significantly decreased, and the tissue characteristics were similar to those of normal WT mice. (red→astrocyte, green→neuronal cells, black→damaged neuronal cells) ( n = 6)

Article Snippet: GDNF Polyclonal antibody , 26179-1-AP , Proteintech.

Techniques: Staining, Labeling, Expressing, Western Blot, Control